Molecular genotyping of Candida species with special respect to Candida (Torulopsis) glabrata strains by arbitrarily primed PCR

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منابع مشابه

Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR.

A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gene fragment was developed by analysis of C. glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.

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The Candida parapsilosis complex is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, and the closely related species Lodderomyces elongisporus. An exon-primed intron-crossing PCR assay was developed here to distinguish the members of the species complex on the basis of the distinct sizes of amplicons, and Candida orthopsilosis and Candida metapsilosis...

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Molecular evaluation of candida species as etiologic agents of onychomycosis

Background and Aim: The aim of the present study is rapid and precise identification of yeast species isolated from nail of patients with clinical features of onychomycosis using PCR-RFLP technique. Methods: 50 patients with involvement of 29 fingernails and 21 toenails were enrolled in the study. Different yeast species were identified by conventional mycological. The PCR-RFLP was tested on...

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In vitro studies of 5-fluorocytosine resistance in Candida albicans and Torulopsis glabrata.

Spontaneous mutants of Candida albicans resistant to 5-fluorocytosine (5-FC) were isolated from a strain susceptible to 5-FC. These mutants were compared with 5-FC-resistant strains of C. albicans and Torulopsis glabrata isolated from patients treated with the drug and from untreated patients. Resistance to 5-FC was in all cases followed by a decreased susceptibility to 5-fluorouracil. Most str...

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Pseudohyphae formation in Candida glabrata due to CO2 exposure

Background and Purpose: Formation of pseudohyphae is considered a virulence factor in Candida species. Generally, Candida glabrata grows as budding yeast cells; however, reports illustrated that C. glabrata could form pseudohyphal cells in response to some stimuli. In this study, we provided insight into the ability of C. glabrata in forming pseudohyphal cells under different levels of carbon d...

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ژورنال

عنوان ژورنال: Journal of Medical Microbiology

سال: 2000

ISSN: 0022-2615,1473-5644

DOI: 10.1099/0022-1317-49-6-575